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  首页 >> 技术支持 >> 克隆载体

 

基因合成通用克隆载体

 

Cloning is free of charge using any of these vectors.

 

名称 大小 抗性 Map 序列 载体特性
pBluescriptII SK(-) 2.9kb Amp PDF Genebank Many cloning RE sites available; Blue/white screen , both BssHII sites flanking the MCS were mutated.
pGS1 2.8kb Amp PDF Genebank pBSK derivative, only SmaI, EcoRV, XhoI sites in MCS; Blue/white screen
pUC19 2.7kb Amp PDF Genebank Many cloning RE sites available; Blue/white screen
pEZseq Kan 2.0kb Kan PDF Genebank Blue/white screen

 

 Note:

   1) Most time Blunt ligation will be used for cloning the synthesized sequences using either SmaI or EcoRV or HicII sites of  these vectors. Please remember to incorparate Restriction Enzyme sites flanking your sequences for your downstream cloning  purpose.

   2) For cloning into other non-standard vectors, such as pET28a, pcDNA4/TO, pEGFP-C1 or pFastBac1, cloning fee ¥800-¥2000/subcloning will be applied dependes on the difficulty of the subcloning.

   3) The PlasMapper web site automatically generates and annotates plasmid maps using only the plasmid DNA sequences as  input.


 载体测序引物:

   All vectors could be sequenced using the M13 Forward and Reverse Primers:

   1) M13 Forward (-41): 5'- CGC CAG GGT TTT CCC AGT CAC GAC
   2) M13 Reverse (-48): 5'- AGC GGA TAA CAA TTT CAC ACA GGA

 

 Other technical assistance, please call or email us.

 

 

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